Brpf1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Brpf1^{tm1a(EUCOMM)Wtsi}
• Name: bromodomain and PHD finger containing, 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433631
• Synonyms: Brpf1^l
• Gene: Brpf1 Location: Chr6:113284098-113301821 bp, + strand Genetic Position: Chr6, 52.75 cM, cytoband E3
• Alliance: Brpf1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Brpf1 gene page

Mutation origin

• Mutant Cell Lines: EPD0069_1_G04, EPD0069_1_G06
• Germline Transmission: Earliest citation of germline transmission: J:165965
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 113293068 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 113294936. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:222304)

Expression

In Mice Carrying this Mutation:	112 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Brpf1 Mutation: 37 strains or lines available

Notes

Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)