Kdm4b^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm4b^{tm1a(EUCOMM)Wtsi}
• Name: lysine (K)-specific demethylase 4B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433704
• Gene: Kdm4b Location: Chr17:56633062-56709870 bp, + strand Genetic Position: Chr17, 29.27 cM
• Alliance: Kdm4b^{tm1a(EUCOMM)Wtsi} page
• IMPC: Kdm4b gene page

Mutation origin

• Mutant Cell Lines: EPD0070_4_B11, EPD0070_4_E10, EPD0070_4_E11, EPD0070_4_F11, EPD0070_4_G10, EPD0070_4_H10
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 56662423 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56663316. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:302280)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Kdm4b Mutation: 54 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 9 reference(s)