Rhd^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rhd^{tm1e(EUCOMM)Wtsi}
• Name: Rh blood group, D antigen; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4433877
• Gene: Rhd Location: Chr4:134591875-134623483 bp, + strand Genetic Position: Chr4, 67.13 cM
• Alliance: Rhd^{tm1e(EUCOMM)Wtsi} page
• IMPC: Rhd gene page

Mutation origin

• Mutant Cell Lines: EPD0158_3_A05, EPD0158_3_A07, EPD0158_3_D08, EPD0158_3_E07, EPD0158_3_F08, EPD0158_3_H05
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 134606667 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Rhd Mutation: 22 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 4 reference(s)