Aspa^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Aspa^{tm1a(EUCOMM)Wtsi}
• Name: aspartoacylase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4434258
• Synonyms: aspa^lacZ
• Gene: Aspa Location: Chr11:73195813-73217677 bp, - strand Genetic Position: Chr11, 45.28 cM, cytoband B4
• Alliance: Aspa^{tm1a(EUCOMM)Wtsi} page
• IMPC: Aspa gene page

Aspa^{tm1a(EUCOMM)Wtsi}/Aspa^{tm1a(EUCOMM)Wtsi} mice (asterisk) are smaller and show gait abnormalities

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0038_4_C09, EPD0038_4_F09, EPD0038_4_H09
• Germline Transmission: Earliest citation of germline transmission: J:172582
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt2 Mutation details: The L1L2_gt2 cassette was inserted at position 73212647 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 73213675. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:172582, J:173534)

Generation of the Aspa^tm1a(EUCOMM)Wtsi and Aspa^tm1c(EUCOMM)Wtsi alleles

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Aspa Mutation: 26 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)