Pik3c2b^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pik3c2b^{tm1e(EUCOMM)Wtsi}
• Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4434406
• Gene: Pik3c2b Location: Chr1:132973410-133036429 bp, + strand Genetic Position: Chr1, 57.75 cM
• IMPC: Pik3c2b gene page

Mutation origin

• Mutant Cell Lines: EPD0435_2_A09, EPD0435_2_C11, EPD0435_2_E12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 132997595 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Pik3c2b Mutation: 82 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)