Pus10^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Pus10^{tm1a(EUCOMM)Hmgu}
• Name: pseudouridylate synthase 10; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434872
• Gene: Pus10 Location: Chr11:23615674-23682876 bp, + strand Genetic Position: Chr11, 14.34 cM, cytoband A3.3
• Alliance: Pus10^{tm1a(EUCOMM)Hmgu} page
• IMPC: Pus10 gene page

Mutation origin

• Mutant Cell Lines: HEPD0527_5_A09, HEPD0527_5_A11, HEPD0527_5_B09, HEPD0527_5_B10, HEPD0527_5_B12, HEPD0527_5_C10, HEPD0527_5_C12, HEPD0527_5_D10, HEPD0527_5_D11, HEPD0527_5_D12, HEPD0527_5_E10, HEPD0527_5_F09, HEPD0527_5_G12, HEPD0527_5_H10
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 23649482 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 23650188. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Pus10 Mutation: 41 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)