Zc3h12a^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zc3h12a^{tm1a(EUCOMM)Hmgu}
• Name: zinc finger CCCH type containing 12A; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434879
• Gene: Zc3h12a Location: Chr4:125012216-125021633 bp, - strand Genetic Position: Chr4, 58.1 cM
• Alliance: Zc3h12a^{tm1a(EUCOMM)Hmgu} page
• IMPC: Zc3h12a gene page

Mutation origin

• Mutant Cell Lines: HEPD0517_2_B05, HEPD0517_2_C04, HEPD0517_2_C06, HEPD0517_2_E05, HEPD0517_2_F06, HEPD0517_2_G04, HEPD0517_2_G05, HEPD0517_2_H05
• Germline Transmission: Earliest citation of germline transmission: J:255787
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 125015788 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 125016657. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Zc3h12a Mutation: 35 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)