Sema3f^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sema3f^{tm1a(EUCOMM)Hmgu}
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435144
• Gene: Sema3f Location: Chr9:107558699-107587674 bp, - strand Genetic Position: Chr9, 58.91 cM, cytoband F2
• Alliance: Sema3f^{tm1a(EUCOMM)Hmgu} page
• IMPC: Sema3f gene page

Mutation origin

• Mutant Cell Lines: HEPD0570_6_A02, HEPD0570_6_A04, HEPD0570_6_B01, HEPD0570_6_B02, HEPD0570_6_B04, HEPD0570_6_C03, HEPD0570_6_D02, HEPD0570_6_D03, HEPD0570_6_D04, HEPD0570_6_E01, HEPD0570_6_E02, HEPD0570_6_E03, HEPD0570_6_F01, HEPD0570_6_F03, HEPD0570_6_G02, HEPD0570_6_G03, HEPD0570_6_H01, HEPD0570_6_H02, HEPD0570_6_H03
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 107569072 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 107570386. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Sema3f Mutation: 41 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)