Pebp4^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Pebp4^{tm1a(EUCOMM)Hmgu}
• Name: phosphatidylethanolamine binding protein 4; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4436202
• Gene: Pebp4 Location: Chr14:70077869-70297367 bp, + strand Genetic Position: Chr14, 36.1 cM, cytoband D1
• Alliance: Pebp4^{tm1a(EUCOMM)Hmgu} page
• IMPC: Pebp4 gene page

Mutation origin

• Mutant Cell Lines: HEPD0526_4_A08, HEPD0526_4_B05, HEPD0526_4_C06, HEPD0526_4_C07, HEPD0526_4_D05, HEPD0526_4_D07, HEPD0526_4_E07, HEPD0526_4_E08, HEPD0526_4_F06, HEPD0526_4_F08, HEPD0526_4_G05, HEPD0526_4_G06, HEPD0526_4_G07, HEPD0526_4_G08, HEPD0526_4_H06, HEPD0526_4_H08
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 70088633 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 70089470. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Pebp4 Mutation: 17 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)