Aicdatm1.1(cre/ERT2)Crey
Targeted Allele Detail
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Symbol: |
Aicdatm1.1(cre/ERT2)Crey |
Name: |
activation-induced cytidine deaminase; targeted mutation 1.1, Claude-Agnes Reynaud |
MGI ID: |
MGI:4437052 |
Synonyms: |
AID-Cre-ERT2 |
Gene: |
Aicda Location: Chr6:122530768-122541139 bp, + strand Genetic Position: Chr6, 57.65 cM, cytoband F2
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Alliance: |
Aicdatm1.1(cre/ERT2)Crey page
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Germline Transmission: |
Earliest citation of germline transmission:
J:157741
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Parent Cell Line: |
E14.1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Inducible, Null/knockout, Recombinase) |
Inducer: |
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tamoxifen |
Mutations: |
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Insertion, Intragenic deletion
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Aicdatm1.1(cre/ERT2)Crey expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A targeting construct was designed to insert a cre/ERT2 sequence, an SV40 polyA site, and a floxed neo selection cassette into the Aicda locus, replacing exon 2 and fusing with sequence encoding the first 4 amino acids, including 4 bases downstream of the exon 2 splice site. The splice acceptor site of Aicda intron 2-3 was mutated to prevent splicing events that could eliminate the cre/ERT2 sequence. E14.1 ES cells were electroporated with the linearized vector. Recombinant clones were transiently transfected with a cre-expressing vector to excise the neo cassette.
(J:157741)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Aicda
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:157741 Dogan I, et al., Multiple layers of B cell memory with different effector functions. Nat Immunol. 2009 Dec;10(12):1292-9 |
All: |
25 reference(s) |
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