Asns^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Asns^{tm1a(EUCOMM)Wtsi}
• Name: asparagine synthetase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441742
• Synonyms: Asns^-
• Gene: Asns Location: Chr6:7675169-7693209 bp, - strand Genetic Position: Chr6, 3.31 cM, cytoband A1
• Alliance: Asns^{tm1a(EUCOMM)Wtsi} page
• IMPC: Asns gene page

Mutation origin

• Mutant Cell Lines: EPD0525_1_A02, EPD0525_1_A03, EPD0525_1_A04, EPD0525_1_B01, EPD0525_1_B02, EPD0525_1_B03, EPD0525_1_C01, EPD0525_1_C02, EPD0525_1_C03, EPD0525_1_D01, EPD0525_1_D02, EPD0525_1_D03, EPD0525_1_E01, EPD0525_1_E02, EPD0525_1_E03, EPD0525_1_E04, EPD0525_1_F01, EPD0525_1_F02, EPD0525_1_F03, EPD0525_1_G01, EPD0525_1_G02, EPD0525_1_G03, EPD0525_1_G04, EPD0525_1_H01, EPD0525_1_H02, EPD0525_1_H03
• Germline Transmission: Earliest citation of germline transmission: J:165965
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 7684884 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 7684884. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Mice Carrying this Mutation:	10 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Asns Mutation: 41 strains or lines available

Notes

Special note about mutant cell line EPD0525_1_F01: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis of adult brains revealed that homozygous mutant mice express ~20% of the mRNA level expressed in wild-type brains (J:236441).

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 8 reference(s)