Gpsm2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gpsm2^{tm1a(EUCOMM)Wtsi}
• Name: G-protein signalling modulator 2 (AGS3-like, C. elegans); targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441912
• Synonyms: LGN betageo; flox, LGN^BF
• Gene: Gpsm2 Location: Chr3:108585954-108629625 bp, - strand Genetic Position: Chr3, 47.53 cM, cytoband F3
• Alliance: Gpsm2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Gpsm2 gene page

Mutation origin

• Mutant Cell Lines: EPD0519_5_A12, EPD0519_5_B12, EPD0519_5_C12, EPD0519_5_E11, EPD0519_5_E12, EPD0519_5_F11, EPD0519_5_G11, EPD0519_5_G12, EPD0519_5_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 108608828 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 108609458. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:214064)

Expression

In Mice Carrying this Mutation:

2 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gpsm2^{tm1a(EUCOMM)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Gpsm2 Mutation: 26 strains or lines available

Notes

Cell line EPD0519_5_E12 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236519

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 14 reference(s)