Cemip2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cemip2^{tm1a(EUCOMM)Wtsi}
• Name: cell migration inducing hyaluronidase 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441917
• Gene: Cemip2 Location: Chr19:21755706-21835724 bp, + strand Genetic Position: Chr19, 15.54 cM
• IMPC: Cemip2 gene page

Mutation origin

• Mutant Cell Lines: EPD0510_2_A11, EPD0510_2_A12, EPD0510_2_B09, EPD0510_2_B11, EPD0510_2_C09, EPD0510_2_C10, EPD0510_2_D10, EPD0510_2_F10, EPD0510_2_G09, EPD0510_2_H12, EPD0664_5_B06, EPD0664_5_C07, EPD0664_5_C08, EPD0664_5_D06, EPD0664_5_E05, EPD0664_5_E08, EPD0664_5_F06, EPD0664_5_F08, EPD0664_5_G05, EPD0664_5_G06, EPD0664_5_G08, EPD0664_5_H06
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 21774252 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 21776056. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cemip2 Mutation: 95 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)