Spata25^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Spata25^{tm1(KOMP)Wtsi}
• Name: spermatogenesis associated 25; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4451280
• Gene: Spata25 Location: Chr2:164669302-164670454 bp, - strand Genetic Position: Chr2, 85.27 cM, cytoband H3
• Alliance: Spata25^{tm1(KOMP)Wtsi} page
• IMPC: Spata25 gene page

Mutation origin

• Mutant Cell Lines: EPD0487_4_A01, EPD0487_4_A02, EPD0487_4_A03, EPD0487_4_A04, EPD0487_4_B01, EPD0487_4_B02, EPD0487_4_B03, EPD0487_4_B04, EPD0487_4_C01, EPD0487_4_C02, EPD0487_4_C03, EPD0487_4_C04, EPD0487_4_D01, EPD0487_4_D02, EPD0487_4_D03, EPD0487_4_D04, EPD0487_4_E01, EPD0487_4_E02, EPD0487_4_E03, EPD0487_4_E04, EPD0487_4_F01, EPD0487_4_F02, EPD0487_4_F03, EPD0487_4_F04, EPD0487_4_G02, EPD0487_4_G03, EPD0487_4_G04, EPD0487_4_H01, EPD0487_4_H02, EPD0487_4_H03, EPD0487_4_H04
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 669 starting at position 164669294 and ending at position 164669963 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Spata25 Mutation: 10 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)