Lacc1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Lacc1^{tm1a(KOMP)Wtsi}
• Name: laccase domain containing 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4451304
• Synonyms: mFamin^-
• Gene: Lacc1 Location: Chr14:77261640-77274344 bp, - strand Genetic Position: Chr14, 40.63 cM
• Alliance: Lacc1^{tm1a(KOMP)Wtsi} page
• IMPC: Lacc1 gene page

Mutation origin

• Mutant Cell Lines: EPD0538_1_A01, EPD0538_1_A02, EPD0538_1_A05, EPD0538_1_B03, EPD0538_1_B04, EPD0538_1_C03, EPD0538_1_C04, EPD0538_1_C05, EPD0538_1_D04, EPD0538_1_D06, EPD0538_1_E03, EPD0538_1_F01, EPD0538_1_F03, EPD0538_1_F04, EPD0538_1_F05, EPD0538_1_G03, EPD0538_1_G04, EPD0538_1_G05, EPD0538_1_G06, EPD0538_1_H01, EPD0538_1_H03, EPD0538_1_H04, EPD0538_1_H06
• Germline Transmission: Earliest citation of germline transmission: J:244365
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 77271161 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77272045. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Lacc1 Mutation: 28 strains or lines available

Notes

Cell line EPD0538_1_D06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:244365

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 8 reference(s)