Spag16tm1e(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Spag16tm1e(EUCOMM)Hmgu |
Name: |
sperm associated antigen 16; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:4451939 |
Gene: |
Spag16 Location: Chr1:69866129-70764291 bp, + strand Genetic Position: Chr1, 35.04 cM, cytoband C3
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IMPC: |
Spag16 gene page |
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Mutant Cell Line: |
HEPD0615_1_F03 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 69897161 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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