Crh^tm1(cre)Zjh
Targeted Allele Detail

Summary

• Symbol: Crh^tm1(cre)Zjh
• Name: corticotropin releasing hormone; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:4452089
• Synonyms: CRF-iCre, CRF-ires-Cre, CRH-iCre, CRH-ires-CRE
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^tm1(cre)Zjh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crh^tm1(cre)Zjh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crh (MGI:88496)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the corticotropin releasing hormone locus (Crh). The mice were bred to actin-Flpe animals to remove the neo cassette and the Flpe transgene was subsequently bred out of the line. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crh (mouse) Summary of all recombinase alleles driven by Crh. Comparative matrix view of recombinase activities Alliance mouse Crh gene page

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 42 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 119 reference(s)