Tmem74b^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem74b^{tm1e(KOMP)Wtsi}
• Name: transmembrane protein 74B; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4455528
• Gene: Tmem74b Location: Chr2:151543928-151554077 bp, + strand Genetic Position: Chr2, 74.83 cM
• Alliance: Tmem74b^{tm1e(KOMP)Wtsi} page
• IMPC: Tmem74b gene page

Mutation origin

• Mutant Cell Lines: EPD0574_5_A03, EPD0574_5_B01, EPD0574_5_B03, EPD0574_5_D01, EPD0574_5_D04, EPD0574_5_E01, EPD0574_5_E03, EPD0574_5_F01
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 151547751 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Tmem74b Mutation: 7 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)