Cyb5r2^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cyb5r2^{tm1a(EUCOMM)Hmgu}
• Name: cytochrome b5 reductase 2; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4455692
• Gene: Cyb5r2 Location: Chr7:107347662-107357239 bp, - strand Genetic Position: Chr7, 57.21 cM
• Alliance: Cyb5r2^{tm1a(EUCOMM)Hmgu} page
• IMPC: Cyb5r2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0634_6_A07, HEPD0634_6_A08, HEPD0634_6_A10, HEPD0634_6_A11, HEPD0634_6_B09, HEPD0634_6_B11, HEPD0634_6_B12, HEPD0634_6_C08, HEPD0634_6_C09, HEPD0634_6_C11, HEPD0634_6_D09, HEPD0634_6_E11, HEPD0634_6_F08, HEPD0634_6_F10, HEPD0634_6_F12, HEPD0634_6_G07, HEPD0634_6_H07, HEPD0634_6_H08, HEPD0634_6_H09, HEPD0634_6_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 107353640 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 107354462. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Cyb5r2 Mutation: 13 strains or lines available

Notes

Cell line HEPD0634_6_C08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:211614

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 3 reference(s)