Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)28-4-3Pmay
Transgene Detail

Summary

• Symbol: Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)28-4-3Pmay
• Name: transgene insertion 28-4-3, Peter Maye
• MGI ID: MGI:4456882
• Synonyms: DMP1-mcherry/IBSP-topaz, Tg(Acp5-ECFP,Ibsp-Topaz,Dmp1-mCherry)28-4-3Pmay
• Transgene: Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)28-4-3Pmay Location: unknown
• Alliance: Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)28-4-3Pmay page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains genes encoding optically distinct fluorescent proteins inserted into each of three mouse genes derived from two bacterial artificial chromosomes (BACs), RP23-412o5 and RP23-395m11. The mouse genes are acid phosphatase 5, tartrate resistant (Acp5/TRAP), integrin binding sialoprotein (Ibsp) and dentin matrix protein 1 (Dmp1). The coding sequence for ECFP was inserted into Acp5, for Topaz into Ibsp and for mCherry into Dmp1. Ibsp-Topaz and Dmp1-mCherry exhibit bright fluorescence in the skull and mandible of newborn mouse pups and in the tail of adult mice. Fluorescence microscopy demonstrates that Ibsp-Topaz is expressed strongly in osteoblasts and weakly in osteocytes and hypertrophic chondrocytes, whereas Dmp1-mCherry is expressed strongly in osteocytes and weakly in subpopulations of osteoblasts and hypertrophic chondrocytes. The Acp5 reporter is expressed, though strongly, only in an occasional cell. (J:160963)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:160963 Maye P, et al., A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs. BMC Biotechnol. 2009;9:20
• All: 2 reference(s)