Decr2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Decr2^{tm1a(EUCOMM)Wtsi}
• Name: 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4460423
• Gene: Decr2 Location: Chr17:26300182-26309096 bp, - strand Genetic Position: Chr17, 13.06 cM, cytoband B1
• Alliance: Decr2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Decr2 gene page

Mutation origin

• Mutant Cell Lines: EPD0594_2_A09, EPD0594_2_A10, EPD0594_2_A11, EPD0594_2_A12, EPD0594_2_B12, EPD0594_2_C10, EPD0594_2_C12, EPD0594_2_D09, EPD0594_2_D10, EPD0594_2_D11, EPD0594_2_D12, EPD0594_2_E09, EPD0594_2_E11, EPD0594_2_E12, EPD0594_2_F09, EPD0594_2_G10, EPD0594_2_G11, EPD0594_2_G12, EPD0594_2_H10, EPD0594_2_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26306052 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26307481. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Decr2 Mutation: 19 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)