Hnrnpr^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hnrnpr^{tm1a(EUCOMM)Wtsi}
• Name: heterogeneous nuclear ribonucleoprotein R; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4820294
• Synonyms: Hnrnpr^tm1a
• Gene: Hnrnpr Location: Chr4:136038253-136086758 bp, + strand Genetic Position: Chr4, 68.4 cM, cytoband D3
• Alliance: Hnrnpr^{tm1a(EUCOMM)Wtsi} page
• IMPC: Hnrnpr gene page

Mutation origin

• Mutant Cell Lines: EPD0609_5_A09, EPD0609_5_A10, EPD0609_5_A11, EPD0609_5_A12, EPD0609_5_B09, EPD0609_5_B10, EPD0609_5_C09, EPD0609_5_C11, EPD0609_5_C12, EPD0609_5_D09, EPD0609_5_D10, EPD0609_5_D11, EPD0609_5_D12, EPD0609_5_E09, EPD0609_5_E10, EPD0609_5_E11, EPD0609_5_F09, EPD0609_5_F12, EPD0609_5_G09, EPD0609_5_G10, EPD0609_5_G12, EPD0609_5_H09, EPD0609_5_H10, EPD0609_5_H11, EPD0609_5_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s), Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 136043191 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 136043993. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Hnrnpr Mutation: 48 strains or lines available

Notes

Alternative splicing of exon 2 of the Hnrnpr pre-mRNA generates two mRNA isoforms with a start codon in either exon 2 or exon 4, giving rise to two protein isoforms that differ at the Nterminus.

Special note about the mutation generated in mutant cell line EPD0609_5_A12: Analysis of protein expression in brain lysates of P5 mice, using an antibody against the C-terminal domain, revealed that the full-isoform of Hnrnpr (hn-RNP R-FL) is absent, while the truncated isoform lacking the N-terminal acidic domain (hnRNP R-deltaN) is still expressed and even increased in homozygous mutant brains by a compensatory mechanism. Absence of hnRNP R-FL isoform and expression of hnRNP R-deltaN is also detectable in the heart and kidney of homozygous mutant mice, without any upregulation of hnRNP R-deltaN in these tissues (J:317395).

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)