Kcnv2^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kcnv2^{tm1(KOMP)Wtsi}
• Name: potassium channel, subfamily V, member 2; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4840969
• Synonyms: Kcnv2^tm1, Kcnv2^{tm1a(KOMP)Wtsi}
• Gene: Kcnv2 Location: Chr19:27299988-27314579 bp, + strand Genetic Position: Chr19, 21.88 cM
• Alliance: Kcnv2^{tm1(KOMP)Wtsi} page
• IMPC: Kcnv2 gene page

Mutation origin

• Mutant Cell Lines: EPD0452_1_E05, EPD0600_3_B03, EPD0600_3_B09, EPD0600_3_B10, EPD0600_3_C08, EPD0600_3_D08, EPD0600_3_F04, EPD0600_3_F06, EPD0600_3_G01, EPD0600_3_H02, EPD0600_3_H04, EPD0600_3_H09
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Del_BactPneo_FFL
• Mutation details: The L1L2_Del_BactPneo_FFL cassette was inserted at position 27299497 of Chromosome 19 upstream of exon 1 (Build GRCm39). The cassette is composed of an FRT site followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a loxP site. A second loxP site is inserted downstream of exon 1 at position 27398917. Exon 1 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/about/targeting-strategies. (J:148605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Kcnv2 Mutation: 24 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)