Skida1^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Skida1^{tm1(KOMP)Wtsi}
• Name: SKI/DACH domain containing 1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4841167
• Gene: Skida1 Location: Chr2:18048510-18053627 bp, - strand Genetic Position: Chr2, 12.83 cM, cytoband A2
• Alliance: Skida1^{tm1(KOMP)Wtsi} page
• IMPC: Skida1 gene page

Mutation origin

• Mutant Cell Lines: EPD0296_3_A09, EPD0296_3_A10, EPD0296_3_A11, EPD0296_3_A12, EPD0296_3_B11, EPD0296_3_C09, EPD0296_3_D09, EPD0296_3_G09, EPD0296_3_G12, EPD0296_3_H09
• Germline Transmission: Earliest citation of germline transmission: J:211773
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4394 starting at position 18048879 and ending at position 18053273 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Skida1 Mutation: 38 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)