Btbd9^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Btbd9^{tm1a(EUCOMM)Wtsi}
• Name: BTB domain containing 9; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842263
• Gene: Btbd9 Location: Chr17:30434498-30795462 bp, - strand Genetic Position: Chr17, 15.62 cM, cytoband B1
• Alliance: Btbd9^{tm1a(EUCOMM)Wtsi} page
• IMPC: Btbd9 gene page

Mutation origin

• Mutant Cell Lines: EPD0596_5_A01, EPD0596_5_A02, EPD0596_5_A03, EPD0596_5_B02, EPD0596_5_B03, EPD0596_5_B04, EPD0596_5_D01, EPD0596_5_D02, EPD0596_5_D03, EPD0596_5_E01, EPD0596_5_E02, EPD0596_5_E03, EPD0596_5_E04, EPD0596_5_F04, EPD0596_5_G01, EPD0596_5_G03, EPD0596_5_H02, EPD0596_5_H03, EPD0596_5_H04, EPD0631_3_A09, EPD0631_3_A11, EPD0631_3_B09, EPD0631_3_B11, EPD0631_3_C10, EPD0631_3_C11, EPD0631_3_C12, EPD0631_3_D11, EPD0631_3_D12, EPD0631_3_E09, EPD0631_3_F12, EPD0631_3_G10, EPD0631_3_G12, EPD0631_3_H09, EPD0631_3_H11
• Germline Transmission: Earliest citation of germline transmission: J:288369
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30743399 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30744374. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Btbd9 Mutation: 51 strains or lines available

Notes

Cell line EPD0631_3_A09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:282582

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)