Bola3^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bola3^{tm1a(EUCOMM)Wtsi}
• Name: bolA family member 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842419
• Synonyms: Bola3^flox
• Gene: Bola3 Location: Chr6:83326129-83337118 bp, + strand Genetic Position: Chr6, 35.94 cM
• Alliance: Bola3^{tm1a(EUCOMM)Wtsi} page
• IMPC: Bola3 gene page

Mutation origin

• Mutant Cell Lines: EPD0618_3_A07, EPD0618_3_A08, EPD0618_3_B05, EPD0618_3_B07, EPD0618_3_C08, EPD0618_3_D05, EPD0618_3_D06, EPD0618_3_E06, EPD0618_3_E07, EPD0618_3_E08, EPD0618_3_F08, EPD0618_3_G08, EPD0618_3_H05, EPD0618_3_H06
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83329588 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 83330358. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Bola3 Mutation: 8 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 4 reference(s)