Tpm1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tpm1^{tm1a(EUCOMM)Wtsi}
• Name: tropomyosin 1, alpha; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842867
• Gene: Tpm1 Location: Chr9:66929872-66956688 bp, - strand Genetic Position: Chr9, 36.27 cM
• Alliance: Tpm1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Tpm1 gene page

Mutation origin

• Mutant Cell Line: EPD0001_3_G07
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: SI2.3 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 66942934 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 66944017. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Tpm1 Mutation: 31 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)