Hbs1l^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hbs1l^{tm1a(KOMP)Wtsi}
• Name: Hbs1-like (S. cerevisiae); targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4887599
• Gene: Hbs1l Location: Chr10:21171876-21244788 bp, + strand Genetic Position: Chr10, 9.75 cM
• Alliance: Hbs1l^{tm1a(KOMP)Wtsi} page
• IMPC: Hbs1l gene page

Mutation origin

• Mutant Cell Lines: EPD0693_2_A02, EPD0693_2_A04, EPD0693_2_B01, EPD0693_2_B03, EPD0693_2_C03, EPD0693_2_G04, EPD0693_2_H01, EPD0693_2_H02, EPD0693_2_H03, EPD0846_6_A01, EPD0846_6_A02, EPD0846_6_A03, EPD0846_6_A04, EPD0846_6_B02, EPD0846_6_B04, EPD0846_6_C01, EPD0846_6_C02, EPD0846_6_C03, EPD0846_6_C04, EPD0846_6_D01, EPD0846_6_D02, EPD0846_6_D03, EPD0846_6_D04, EPD0846_6_E01, EPD0846_6_E02, EPD0846_6_E03, EPD0846_6_E04, EPD0846_6_F01, EPD0846_6_F04, EPD0846_6_G01, EPD0846_6_G02, EPD0846_6_G03, EPD0846_6_G04, EPD0846_6_H03, EPD0846_6_H04
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Modified isoform(s), Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 21212311 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 21213197. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:272028)

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Hbs1l Mutation: 51 strains or lines available

Notes

Special note about mutant cell line EPD0693_2_A02: The mutation generated in this ES cell line results in a hypomorphic allele. RT-PCR analysis revealed a 10% residual expression of mRNA in E9.5 stage homozygous mutant embryos. Western blot analysis confirmed residual expression at the expected band size for HBS1L-V3 in various tissues, with some residual expression of V1/V2 (J:272028).

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 9 reference(s)