Csf2/Il3^{tm1.1(CSF2,IL3)Flv}
Targeted Allele Detail

Summary

• Symbol: Csf2/Il3^{tm1.1(CSF2,IL3)Flv}
• Name: interleukin 3; targeted mutation 1.1, Richard A Flavell
• MGI ID: MGI:4887881
• Gene: Il3 Location: Chr11:54156129-54158103 bp, - strand Genetic Position: Chr11, 32.13 cM, cytoband B1
• Alliance: Csf2/Il3^{tm1.1(CSF2,IL3)Flv} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168302
• Parent Cell Line: V17 (ES Cell)
• Strain of Origin: BALB/cAnNTac x 129S4/SvJae

Mutation description

Allele Type: Targeted (Humanized sequence, Inserted expressed sequence) Mutations: Insertion, Intergenic deletion Mutation details: The coding region of Il3 through Csf2 was replaced with the corresponding human sequence. The human Il3 remains under the control of the mouse promoter while expression of Csf2 is controlled by the human promoter. Cre-mediated recombination removed the selection cassettes. (J:168302)

Strategy to generate the Csf2/Il3^tm1.1(CSF2,IL3)Flv allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il3 Mutation: 31 strains or lines available

Notes

Originally generated in ES cells containing Il2rg^tm1.1Flv and Rag2^tm1.1Flv.

References

• Original: J:168302 Willinger T, et al., Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2390-5
• All: 16 reference(s)