Scube3^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Scube3^tm1Dgen
• Name: signal peptide, CUB domain, EGF-like 3; targeted mutation 1, Deltagen
• MGI ID: MGI:4939493
• Synonyms: Scube3^tm1Dge/H
• Gene: Scube3 Location: Chr17:28361115-28393828 bp, + strand Genetic Position: Chr17, 14.63 cM
• Alliance: Scube3^tm1Dgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168645
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3, encoding the first two EGF-like domains, were replaced with a lacZ and neo cassette. Spatiotemporal expression of lacZ, as determined by X-gal staining of whole and sections of embryos, is reported to resemble closely that previously reported for mRNA expression from the wild-type gene. Western blot analysis detects a truncated protein in homozygous mutant mice. Wholemount immunohistochemical analysis employing an antibody to an epitope in the spacer region, which is encoded by sequence downstream of the cassette insertion site, stains membranes of wild-type, but not of mutant epithelial cells. (J:168645)

Expression

In Mice Carrying this Mutation:

149 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Scube3 Mutation: 47 strains or lines available

References

• Original: J:168645 Xavier GM, et al., Characterization of a mouse Scube3 reporter line. Genesis. 2010 Dec;48(12):684-92
• All: 1 reference(s)