Rilpl1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
|
Symbol: |
Rilpl1tm1a(EUCOMM)Hmgu |
Name: |
Rab interacting lysosomal protein-like 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:4946743 |
Gene: |
Rilpl1 Location: Chr5:124631143-124669454 bp, - strand Genetic Position: Chr5, 63.62 cM, cytoband F
|
Alliance: |
Rilpl1tm1a(EUCOMM)Hmgu page
|
IMPC: |
Rilpl1 gene page |
|
Mutant Cell Lines: |
HEPD0718_1_A05, HEPD0718_1_A06, HEPD0718_1_A08, HEPD0718_1_B05, HEPD0718_1_B06, HEPD0718_1_B07, HEPD0718_1_B08, HEPD0718_1_C06, HEPD0718_1_C07, HEPD0718_1_C08, HEPD0718_1_D06, HEPD0718_1_D07, HEPD0718_1_E06, HEPD0718_1_E08, HEPD0718_1_F06, HEPD0718_1_F07, HEPD0718_1_F08, HEPD0718_1_G06, HEPD0718_1_G07, HEPD0718_1_G08, HEPD0718_1_H05, HEPD0718_1_H06, HEPD0718_1_H07, HEPD0718_1_H08 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
|
Parent Cell Line: |
JM8A3.N1 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 124659047 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 124659884. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
|
|
|
|
Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
2 reference(s) |
|