Nt5c1b^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nt5c1b^{tm1e(KOMP)Wtsi}
• Name: 5'-nucleotidase, cytosolic IB; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:5007968
• Gene: Nt5c1b Location: Chr12:10419973-10440175 bp, + strand Genetic Position: Chr12, 5.27 cM, cytoband A2
• Alliance: Nt5c1b^{tm1e(KOMP)Wtsi} page
• IMPC: Nt5c1b gene page

Mutation origin

• Mutant Cell Lines: EPD0739_2_A09, EPD0739_2_A11, EPD0739_2_C10, EPD0739_2_C11, EPD0739_2_D10, EPD0739_2_D12
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 10424356 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Nt5c1b Mutation: 37 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)