Tg(Igh-6H4Vh/Ighm*a)508Zbz
Transgene Detail

Summary

• Symbol: Tg(Igh-6H4Vh/Ighm*a)508Zbz
• Name: transgene insertion 508, Universitatsspital Zurich, Biologisches Zentrallaboratorium
• MGI ID: MGI:5014082
• Synonyms: 6H4mu, Tg(6H4u)508, TgN(6H4IgH)508
• Transgene: Tg(Igh-6H4Vh/Ighm*a)508Zbz Location: unknown
• Alliance: Tg(Igh-6H4Vh/Ighm*a)508Zbz page

Transgene origin

• Strain of Origin: B6;129S7-Prnp^tm1Cwe

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igh-6H4Vh/Ighm*a)508Zbz expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igh (MGI:96442)
  mouse	Ighm (MGI:96448)

• Mutation details: This transgene encodes an immunoglobulin heavy chain (IgH) whose variable region is that of the monoclonal antibody expressed by the hybridoma cell line 6H4, which recognizes the normal endogenous mouse prion protein (PRNP); this is joined to the IgH-mu constant region (Cu) of the IgH^a haplotype. Transgenic mice develop high titres of anti-PRNP antibodies composed of transgene-derived IgM^a mu heavy chains paired with various endogenously encoded immunoglobulin kappa and lambda light chains, but have no detectable IgHM/D from endogenous genes. (J:127850)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Six mice that had integrated this transgenic construct were designated 508-511, 513 and 514. Four of these mice (unspecified) expressed the transgene. Mice descended from founder 508 - the present line - were employed in all experiments described. (J:127850)

References

• Original: J:127850 Heppner FL, et al., Prevention of scrapie pathogenesis by transgenic expression of anti-prion protein antibodies. Science. 2001 Oct 5;294(5540):178-82
• All: 1 reference(s)