Tg(Pcp2-Prnp)F332Zbz
Transgene Detail
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Symbol: |
Tg(Pcp2-Prnp)F332Zbz |
Name: |
transgene insertion F332, Zurich |
MGI ID: |
MGI:5014403 |
Synonyms: |
TgF32, Tg L7-deltaPrP, Tg(L7-Prnp)F332, Tg(PrPdelta134)332Zbz |
Transgene: |
Tg(Pcp2-Prnp)F332Zbz Location: unknown
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Alliance: |
Tg(Pcp2-Prnp)F332Zbz page
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(Pcp2-Prnp)F332Zbz expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Note |
mouse |
Prnp (MGI:97769) |
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Mutation details: Mice bearing this transgene express a truncated mouse prion protein (PRNP) lacking amino acids 32 through 134 under the transcriptional control of the regulatory elements of the Purkinje cell protein 2 (L7) gene. A cDNA encoding the modified PRNP and extending to the EcoR1 site in the 3' UTR was inserted into a cloned mouse genomic DNA segment containing the entire Pcp2 coding region, 4 kb of 5' flanking sequence and 2 kb of 3' flanking sequence. Insertion occurred at the BamH1 site in the fourth (third coding) exon, so the initial translation product has 80 amino acids of PCP2/L7 joined to its amino terminus; these presumably are removed along with the PRNP N-terminal leader sequence. Quantitative PCR analysis suggests this line carries ~130 transgene copies. Immunohistochemical analysis demonstrated high levels of expression of the truncated PRNP in cell bodies, axons and dendrites only of Purkinje cells of transgenic mice lacking endogenous PRNP expression; no expression is seen in granule cells.
(J:47034, J:83952, J:173517)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Three transgenic lines of mice were generated and designated F13, F332 (aka F32) and F337 (aka F37). ( J:83952)
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Original: |
J:83952 Flechsig E, et al., Expression of truncated PrP targeted to Purkinje cells of PrP knockout mice causes Purkinje cell death and ataxia. EMBO J. 2003 Jun 16;22(12):3095-101 |
All: |
3 reference(s) |
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