Chka^{tm2a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Chka^{tm2a(KOMP)Wtsi}
• Name: choline kinase alpha; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5050150
• Gene: Chka Location: Chr19:3901773-3944369 bp, + strand Genetic Position: Chr19, 3.57 cM
• Alliance: Chka^{tm2a(KOMP)Wtsi} page
• IMPC: Chka gene page

Mutation origin

• Mutant Cell Lines: EPD0815_8_A01, EPD0815_8_A03, EPD0815_8_A04, EPD0815_8_B01, EPD0815_8_F01, EPD0815_8_G03, EPD0815_8_G04, EPD0815_8_H03
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 3934200 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 3937607. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Chka Mutation: 30 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)