Tg(Ckm-Dmd_iDp71)MCA-1Chmb
Transgene Detail

Summary

• Symbol: Tg(Ckm-Dmd_iDp71)MCA-1Chmb
• Name: transgene insertion MCA-1, Jeffrey S Chamberlain
• MGI ID: MGI:5140740
• Synonyms: Dp71, MCA-1
• Transgene: Tg(Ckm-Dmd_iDp71)MCA-1Chmb Location: unknown
• Alliance: Tg(Ckm-Dmd_iDp71)MCA-1Chmb page

Transgene origin

• Strain of Origin: C57BL/6J x SJL/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-Dmd_iDp71)MCA-1Chmb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dmd (MGI:94909)

• Mutation details: The mouse creatine kinase, muscle (Ckm) regulatory sequences, including 3,300 bp of 5' flanking sequence, exon 1, intron 1 and a truncated exon 2 ending just upstream of the translation initiation codon, drives expression in muscle of a cDNA containing exons 63 through 79 of the mouse dystrophin, muscular dystrophy (Dmd) gene. This represents the non-muscle Dp71 dystrophin mRNA isoform that begins at the unique Dp71 exon containing the 5' non-coding region and N-terminal coding sequence; the protein it encodes (sometimes called apo-dystrophin 1) contains only the cysteine-rich and C-terminal dystrophin associated protein complex (DAPC) interacting domains. Immunoblot analysis demonstrates expression of the Dp71 protein isoform in skeletal and cardiac muscle of transgenic, but not of control, mice. Immunohistochemical analysis shows its expression in skeletal muscle at a level similar to that of endogenous muscle dystrophin in control mice and demonstrates its correct localization to the sarcolemmal membrane and concentration at the neuromuscular junction (NMJ). (J:21841)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This transgene is autosomally integrated.

References

• Original: J:21841 Cox GA, et al., Dp71 can restore the dystrophin-associated glycoprotein complex in muscle but fails to prevent dystrophy [see comments]. Nat Genet. 1994 Dec;8(4):333-9
• All: 3 reference(s)