Tg(Ckm-Dmd*)11956Chmb
Transgene Detail

Summary

• Symbol: Tg(Ckm-Dmd*)11956Chmb
• Name: transgene insertion 11956, Jeffrey S Chamberlain
• MGI ID: MGI:5140820
• Synonyms: 11956 CVBA3', 11956CVBA3', delta17-48, Tg(CVBA3)Ked
• Transgene: Tg(Ckm-Dmd*)11956Chmb Location: unknown
• Alliance: Tg(Ckm-Dmd*)11956Chmb page

Transgene origin

• Strain of Origin: C57BL/6J x SJL/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-Dmd*)11956Chmb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dmd (MGI:94909)

• Mutation details: Mice with this transgene express an internally deleted mouse dystrophin protein under direction of the muscle specific enhancer and promoter of the mouse creatine kinase, muscle (Ckm) gene. The transgene contains nucleotides -3300 through +7 from Ckm followed by the SV40 VP1 intron, the dystrophin cDNA and the SV40 late polyadenylation signal. The dystrophin, muscular dystrophy (Dmd) cDNA includes the full length 5' and 3' untranslated regions (UTRs) and contains a deletion mimicking a human Becker muscular dystrophy mutation associated with a very mild phenotype; this deletion encompasses 5,106 base pairs of the coding region, derived from exons 17-48, of the full length muscle mRNA isoform, comprising nearly half the coding sequence. Immunoblot analysis demonstrates uniform expression of the truncated protein in quadriceps at >10-fold and in diaphragm at 0.9-fold the the level of full length dystrophin in the corresponding muscles of wild-type mice. (J:27471)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:27471 Phelps SF, et al., Expression of full-length and truncated dystrophin mini-genes in transgenic mdx mice. Hum Mol Genet. 1995 Aug;4(8):1251-8
• All: 4 reference(s)