Ldhb^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ldhb^{tm1a(KOMP)Wtsi}
• Name: lactate dehydrogenase B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5299501
• Gene: Ldhb Location: Chr6:142435975-142453683 bp, - strand Genetic Position: Chr6, 74.17 cM
• Alliance: Ldhb^{tm1a(KOMP)Wtsi} page
• IMPC: Ldhb gene page

Mutation origin

• Mutant Cell Lines: EPD0016_1_A10, EPD0016_1_A11, EPD0016_1_E10, EPD0016_1_E11, EPD0016_1_G12, EPD0017_2_C05, EPD0017_2_E06, EPD0017_2_F04, EPD0017_2_G06
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 142446821 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 142447622. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Ldhb Mutation: 34 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)