Dnmt3l^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dnmt3l^{tm1a(EUCOMM)Wtsi}
• Name: DNA methyltransferase 3-like; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5302375
• Gene: Dnmt3l Location: Chr10:77878121-77899456 bp, + strand Genetic Position: Chr10, 39.72 cM
• IMPC: Dnmt3l gene page

Mutation origin

• Mutant Cell Lines: EPD0013_1_B06, EPD0013_1_C06, EPD0013_1_D06, EPD0013_1_F06, EPD0022_1_B01, EPD0022_1_B02, EPD0022_1_C02, EPD0022_1_E01, EPD0022_1_E03, EPD0022_1_F01, EPD0022_1_F03
• Germline Transmission: Unknown
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 77890241 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 77891208. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3l Mutation: 52 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)