Tg(CD2-Ptpn11*C459S)1Axdr
Transgene Detail

Summary

• Symbol: Tg(CD2-Ptpn11*C459S)1Axdr
• Name: transgene insertion 1, Denis R Alexander
• MGI ID: MGI:5310938
• Synonyms: CS-SHP-2, SHP2(CS), Shp2CS, SHP2(CS) L1
• Transgene: Tg(CD2-Ptpn11*C459S)1Axdr Location: unknown
• Alliance: Tg(CD2-Ptpn11*C459S)1Axdr page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A cDNA encoding the major transcript of mouse protein tyrosine phosphatase, non-receptor type 11 (Ptpn11; also called Shp2, src homology 2 domain-containing tyrosine phosphatase 2) with the cysteine at amino acid position 459 replaced by serine (C450S) and an N-terminal polyglutamine tag was inserted into the VA hCD2 expression cassette between the promoter and the 3' locus control region (LCR) of the human CD2 gene. Lysates of T cells, but not of B cells, from mice of this line react with antibody to polyglutamine; antibody-reactive PTPN11/SHP2 is ~7-fold higher in T-cells of transgenic than of wild-type mice. Similar expression is reported in thymus lysates. Despite a marked increase in GAB2 association of both transgene-encoded mutant and endogenous wild-type PTPN11/SHP2 in transgenic T-cells under both basal and stimulated conditions, the increase in PTPase activity that occurs in stimulated wild-type T-cells is completely abrogated, demonstrating a dominant-negative, inhibitory effect of the mutant enzyme on the activity of the wild-type enzyme. (J:105228, J:119396)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Three independent transgenic mouse lines, designated L1, L4 and L5, were established by backcrossing the three germline-transmitting founder mice to B10.BR mice.

References

• Original: J:119396 Salmond RJ, et al., The src homology 2 domain-containing tyrosine phosphatase 2 regulates primary T-dependent immune responses and Th cell differentiation. J Immunol. 2005 Nov 15;175(10):6498-508
• All: 2 reference(s)