Mir182^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir182^tm1Wtsi
• Name: microRNA 182; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320043
• Synonyms: Mir182^ko, Mir182^{tm1(mirKO)Wtsi}
• Gene: Mir182 Location: Chr6:30165917-30165991 bp, - strand Genetic Position: Chr6, 12.52 cM
• Alliance: Mir182^tm1Wtsi page

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_2H7, mirKO_ES_PuDtk_2H8, mirKO_ES_PuDtk_2H9, mirKO_ES_PuDtk_2H10, mirKO_ES_PuDtk_2H11, mirKO_ES_PuDtk_2H12
• Germline Transmission: Earliest citation of germline transmission: J:303596
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: A PGK_EM7_PuDtk_bGHpA cassette replaced a genomic region of size 190 starting at position 30115899 and ending at position 30116090 of Chromosome 6 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Expression

In Mice Carrying this Mutation:

104036 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mir182 Mutation: 8 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)