Mir154tm1Wtsi
Targeted Allele Detail
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Symbol: |
Mir154tm1Wtsi |
Name: |
microRNA 154; targeted mutation 1, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5320100 |
Synonyms: |
Mir154tm1(mirKO)Wtsi |
Gene: |
Mir154 Location: Chr12:109704867-109704932 bp, + strand Genetic Position: Chr12, 60.56 cM
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Mutant Cell Lines: |
mirKO_ES_PuDtk_16B1, mirKO_ES_PuDtk_16B2, mirKO_ES_PuDtk_16B3 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
Sanger miRNA knockouts
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
Vector: PGK_EM7_PuDtk_bGHpA
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Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 171 starting at position 109704797 and ending at position 109704968 of Chromosome 12 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site.
(J:174268)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Mir154 Mutation: |
0 strains or lines available
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Original: |
J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5 |
All: |
1 reference(s) |
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