Esr1^tm1.1Gust
Targeted Allele Detail

Summary

• Symbol: Esr1^tm1.1Gust
• Name: estrogen receptor 1 (alpha); targeted mutation 1.1, Jan-Ake Gustafsson
• MGI ID: MGI:5433576
• Synonyms: ERalpha^-
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm1.1Gust page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186230
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3 and a loxP site and FRT flanked neo cassette were inserted downstream of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination removed exon 3. Western blot analysis confirmed the absence of expression in ovary, mammary gland and uterus tissues from homozygous mice. (J:186230)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 68 strains or lines available

References

• Original: J:186230 Antonson P, et al., Generation of ERalpha-floxed and knockout mice using the Cre/LoxP system. Biochem Biophys Res Commun. 2012 Aug 10;424(4):710-6
• All: 1 reference(s)