Tg(Slc16a1-luc)50Rttr
Transgene Detail

Summary

• Symbol: Tg(Slc16a1-luc)50Rttr
• Name: transgene insertion 50, Guy Rutter
• MGI ID: MGI:5465430
• Synonyms: Mct1-Luc
• Transgene: Tg(Slc16a1-luc)50Rttr Location: unknown
• Alliance: Tg(Slc16a1-luc)50Rttr page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The murine Mct1 coding sequence was amplified from pUC18-Mct1, with the addition of a single COOH-terminal c-Myc epitope tag by PCR (primer sequences in Supplementary Table 1), and inserted between the NheI and EcoRV sites of plasmid pBI-L Tet (Clontech). This generated a plasmid with a bidirectional tetracycline-regulated promoter driving expression of both Mct1::Myc and firefly luciferase. Lines 28, 50 and 60 were generated. (J:192583)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:192583 Pullen TJ, et al., Overexpression of monocarboxylate transporter-1 (SLC16A1) in mouse pancreatic beta-cells leads to relative hyperinsulinism during exercise. Diabetes. 2012 Jul;61(7):1719-25
• All: 1 reference(s)