Igs5^{tm2.1(ACTB-EGFP,-tdTomato)Luo}
Targeted Allele Detail

Summary

• Symbol: Igs5^{tm2.1(ACTB-EGFP,-tdTomato)Luo}
• Name: intergenic site 5; targeted mutation 2.1, Liqun Luo
• MGI ID: MGI:5470395
• Synonyms: Iis5^{tm2.1(ACTB-EGFP,-tdTomato)Luo}, MADM-7^GT
• Gene: Igs5 Location: unknown Genetic Position: Chr7, 2.13 cM
• Alliance: Igs5^{tm2.1(ACTB-EGFP,-tdTomato)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194079
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM. Mice were bred to Hprt-Cre mice (on an undisclosed genetic background) and the floxed neomycin resistance gene was removed. (J:194079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs5 Mutation: 4 strains or lines available

References

• Original: J:194079 Hippenmeyer S, et al., Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance. Cell Rep. 2013 Mar 28;3(3):960-7
• All: 9 reference(s)