Chat^tm2(cre)Lowl
Targeted Allele Detail

Summary

• Symbol: Chat^tm2(cre)Lowl
• Name: choline O-acetyltransferase; targeted mutation 2, Bradford B Lowell
• MGI ID: MGI:5475195
• Synonyms: ChAT-IRES-Cre
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat^tm2(cre)Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169562
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Chat^tm2(cre)Lowl expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Chat (MGI:88392)

• Mutation details: An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chat^tm1(cre)Lowl. (J:169562)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Chat (mouse) Summary of all recombinase alleles driven by Chat. Comparative matrix view of recombinase activities Alliance mouse Chat gene page

Disease models

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:169562 Rossi J, et al., Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab. 2011 Feb 2;13(2):195-204
• All: 383 reference(s)