Hprt1^{tm1(Cd68-BCL2)Ple}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Cd68-BCL2)Ple}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Philippe Lesnik
• MGI ID: MGI:5475555
• Synonyms: Mphi-hBcl-2, Tg CD68 hBcl2
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Cd68-BCL2)Ple} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166498
• Parent Cell Line: BPES (ES Cell)
• Strain of Origin: (129-Hprt1^b-m3 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^{tm1(Cd68-BCL2)Ple} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2 (596)

  Bcl2	(MGI:88138)

• Mutation details: This single-copy, targeted insertion into the Hprt locus consists of the macrophage-specific mouse Cd68 promoter followed by a cDNA encoding the human B cell leukemia/lymphoma 2 protein and the mouse EF1 polyadenylation signal; the vector also contains sequence that repairs an inactivating deletion in the Hprt gene of the ES cell line. Human BCL2 mRNA and protein are detected by reverse transcriptase/polymerase chain reaction (RT-PCR) and flow cytometry, respectively, of thioglycollate-elicited peritoneal macrophages from transgenic mice. (J:166498)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:166498 Gautier EL, et al., Macrophage apoptosis exerts divergent effects on atherogenesis as a function of lesion stage. Circulation. 2009 Apr 7;119(13):1795-804
• All: 3 reference(s)