Igs6^{tm2.1(ACTB-EFGP,-tdTomato)Luo}
Targeted Allele Detail

Summary

• Symbol: Igs6^{tm2.1(ACTB-EFGP,-tdTomato)Luo}
• Name: intergenic site 6; targeted mutation 2.1, Liqun Luo
• MGI ID: MGI:5491599
• Synonyms: Iis6^{tm2.1(ACTB-EFGP,-tdTomato)Luo}, John12-GT, John12^GT, M12-GT, MADM-12-GT, MADM-12^GT, MADM-12GT, MADM-12^TG
• Gene: Igs6 Location: unknown Genetic Position: Chr12, 1.71 cM
• Alliance: Igs6^{tm2.1(ACTB-EFGP,-tdTomato)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194079
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM" cassette. The "GT MADM" cassette used here was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire "GT MADM" construct was inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). Cre-mediated recombination removed the floxed neo cassette. (J:194079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs6 Mutation: 4 strains or lines available

References

• Original: J:194079 Hippenmeyer S, et al., Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance. Cell Rep. 2013 Mar 28;3(3):960-7
• All: 3 reference(s)