Igs6^{tm1.1(ACTB-tdTomato,-EFGP)Luo}
Targeted Allele Detail

Summary

• Symbol: Igs6^{tm1.1(ACTB-tdTomato,-EFGP)Luo}
• Name: intergenic site 6; targeted mutation 1.1, Liqun Luo
• MGI ID: MGI:5491600
• Synonyms: Iis6^{tm1.1(ACTB-tdTomato,-EFGP)Luo}, John12-TG, John12^TG, M12-TG, MADM-12-TG, MADM-12^TG, MADM-12^TG, MADM-12TG
• Gene: Igs6 Location: unknown Genetic Position: Chr12, 1.71 cM
• Alliance: Igs6^{tm1.1(ACTB-tdTomato,-EFGP)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194079
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Iis6 locus located about 16 kb downstream of exon 1 of the Rab10 gene at 1.71 cM. Cre-mediated recombination removed the neo cassette. (J:194079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs6 Mutation: 4 strains or lines available

References

• Original: J:194079 Hippenmeyer S, et al., Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance. Cell Rep. 2013 Mar 28;3(3):960-7
• All: 3 reference(s)