Prkab1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prkab1^{tm1b(KOMP)Wtsi}
• Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5501071
• Synonyms: Prkab1^tm1b
• Gene: Prkab1 Location: Chr5:116151654-116162449 bp, - strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Prkab1^{tm1b(KOMP)Wtsi} page
• IMPC: Prkab1 gene page

Mutation origin

• Mutant Cell Line: EPD0033_3_C09
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 116159303 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site was inserted downstream of the targeted exon(s) at position 116160137. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision removed the critical sequence and neomycin selection cassette yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	1047 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Prkab1 Mutation: 24 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 8 reference(s)